Page 14 - N. Metabolism and metabolic diseases

[N. Metabolism and metabolic diseases-7]

Anti-osteoclastogenic activity of hibiscus syriacus L. twig

extract via inhibition of ERK/Akt-c-Fos-NFATc1 signaling axis

Shin-Hye Kim¹˙², Sik-Won Choi¹

¹Forest Biomaterials Research Center, National Institute of Forest Science(NIFS), Jinju 52817, Korea, ²Department of

Biological Sciences, Chonbuk National University, Jeonju 54896, Korea

A decrease of bone mass is a major risk factor for fracture. Several natural products have traditionally been used
as herbal medicines to prevent and/or treat bone disorders including osteoporosis. Hibiscus syriacus has several

biological activities including anti-osteoclasts, but its anti-osteoporotic activity has not been fully studied yet. The

effect of hibiscus syriacus L. cultivars stem with color of flowers on the differentiation of bone marrow-derived
macrophages into osteoclasts was examined by phenotype assay and confirmed by real-time PCR and
immunoblotting. Interestingly, the branch extracts of purple flowers with red eyes strongly inhibited the RANKL-

stimulated osteoclast differentiation compared with other varieties. In mode of action, the purple flower cultivar,
‘Nanpa’ extracts attenuated the RANKL-induced osteoclast differentiation and induction of c-Fos/NFATc1 such as

master regulator of osteoclastogenesis. Moreover, ‘Nanpa’ extracts blocked the RANKL-mediated activation of
AKT/ERK phosphorylation in BMMs. Taken together; our results suggest that the cultivar of purple flowers with red

eyes, ‘Nanpa’ branch extracts could exhibit its anti-osteoclastogenic activity by inhibiting AKT/ERK-c-Fos-NFATc1
signaling molecules