Page 18 - N. Metabolism and metabolic diseases

[N. Metabolism and metabolic diseases-9]

The Regulatory function of Hes1 in metabolic disease related

macrophage

Da-Hyun Kim¹˙², Kyung-Hee Chun¹˙²

¹Department of Biochemistry & Molecular Biology, Yonsei University College of Medicine, seoul 03722, Korea,

²Brain Korea 21 PLUS Project for Medical Science, Yonsei University, seoul 03722, Korea

The central role of adipose tissue in homeostasis became apparent during the development of obesity. Immune

cells in adipose tissues such as macrophages have gained much attention as an important contributor in regulatory
functioning, but its contribution to obesity-related metabolic diseases remained unknown. Notch signaling is
involved in various aspects of cellular regulation. It’s been known that genetic ablation of several key components

of the Notch pathway fails to differentiate adipocytes. Hes1, which is a member of Notch family, can be modulated
by innate inflammatory signals and since Hes1 negatively regulates macrophage TLR responses, this expands the

role of Hes1 in immunoregulation suggesting that Hes1 is involved in inflammatory disorders. In this study, Hes1
macrophage specifically knockout mice was acquired by mating Hes1 floxed mice and LysM cre mice. When they

were fed with high fat diet to induce obesity, cKO mice had lesser body weight increase and lesser macrophages
in adipose tissues. In addition, when immune cells were isolated from adipose tissue and analyzed their subsets,

macrophage portion was the most increased immune cell among the others. According to these results, such kind
of results seems to be due to the existence of Hes1 in macrophage.