Page 10 - X. Stem cell biology

[X. Stem cell biology-7]

Differentiation and establishment of epithelial-like cells

derived from human induced pluripotent stem cells by dental

pulp epithelial stem cells

Mi Jang¹˙#, Min-Gi Ki¹˙#, Jihye Yang¹, Ji-Hye Kim¹, Dae-Hyun Jeon¹, Gene Lee¹˙*

¹Seoul National University, School of Dentistry and Dental Reserch Institute, Seoul 03080, Korea

Tooth development and regeneration essentially require interaction between epithelial and ectodermal
mesenchymal stem cells. Most studies of tooth development have a problem of securing resource of human
epithelial stem cells. Recently, we reported novel epithelial stem cell lines derived from human pluripotent stem

cells using Hertwig’s epithelial root sheath/Epithelial rests of Malassez (HERS/ERM) cell line. To confirm the reported
epithelial stem cell establishment system, we attempted another dental epithelial resource instead of HERS/ERM for

differentiation into the epithelial cell. In this study, human induced pluripotent stem cells (hiPSCs) were cocultured
on dental pulp epithelial stem cells (DPESC) isolated from the deciduous tooth. Differentiated hiPSCs showed the

morphological change as epithelial-like shape. Differentiated epithelial-like cells on DPESC (dEPI-iPSC) were
immortalized with SV40 large T antigen to overcome a rare population. Immortalized dEPI-iPSC cell line had a

normal karyotype, and short tandem repeat (STR) analysis verified that it was derived from hiPSCs. A similar protein
expression pattern was detected in dEPI-iPSC and DPESC. These results indicated that epithelial-like cell lines from

hiPSCs were successfully established by DPESCs.