Page 8 - X. Stem cell biology

[X. Stem cell biology-6]

Advanced Strategy for Production of Urine Stem Cells (USCs)

and iPSC-Derived Immune Cells

Kyeongseok Kim¹, Ahmed Dayem Abdal¹, Soo Bin Lee¹, Geun-Ho Kang¹, Kyung Min Lim¹, Se Jong Kim¹,
Jaekwon Seok¹, Hee Jeong Kwak¹, Soo Bin Jang¹, Yu Jin Choi¹, Yoon Joo Lee¹, Polash Kumar Biswas¹,

Ssang-Goo Cho¹

¹Department of Stem Cell & Regenerative Biotechnology and Incurable Disease Animal Model and Stem Cel,
Konkuk University, Seoul 05029, Rep. of KOREA

Urine-derived stem cells (USCs) are considered as desirable cell sources for cell therapy because donor-specific
USCs are easily and non-invasively obtained from urine. Efficient isolation, expansion, and differentiation methods
of USCs are necessary to increase their availability. Here, We developed a method for efficient isolation and

expansion of USCs using Matrigel, and the ROCK inhibitor, Y-27632. The prepared USCs showed significantly
enhanced migration, colony forming capacity, and differentiation into osteogenic or chondrogenic lineage. The

USCs were successfully reprogramed into induced pluripotent stem cells (USC-iPSCs) and further differentiated into
kidney organoid and hematopoietic progenitor cells (HPCs). 3,2′-DHF-treated hiPSCs showed upregulation of

intracellular glutathione (GSH) and an increase in the percentage of GSH-high cells in an analysis with a FreSHtracer
system. Interestingly, culture of the 3,2′-DHF-treated hiPSCs in differentiation media enhanced their mesodermal

differentiation and differentiation into CD34+ CD45+ hematopoietic progenitor cells (HPC) and natural killer cells
(NK) cells.