Page 6 - O. Microbiology
P. 6
[O. Microbiology-8]
Production of recombinant human insulin from transformed
Escherichia coli
Bomin Jang¹, Eunju Im¹, Yeh-Jin Ahn¹˙*
¹Biotechnology, Sangmyung University, Seoul 03016, Korea
Insulin is one of the most industrially valuable recombinant proteins. In this study, we produced recombinant human
insulin from genetically engineered Escherichia coli (E. coli BL21, DE3) that heterologously expressed carrot (Daucus
carota L.) heat shock protein 70 (Hsp70). The proinsulin, B chain, and A chain portions of insulin were separately
cloned into the pVFT2S-6xhis-GST expression vector and expressed by isopropyl β-D-1-thiogalactopyranoside
treatment. Ni-affinity chromatography was used to purify 6xHis-GST tagged insulin. As a result, expression of the
insulin B chain was improved approximately 19-fold higher in the transgenic cell lines than that of control cell lines.
Our result suggests a possible use of plant Hsps to increase recombinant protein production in transgenic E. coli.
By using transgenic E. coli expressing plant heat shock protein, the production of recombinant proteins including
insulin, can be improved.
