Page 4 - O. Microbiology

[O. Microbiology-4]

The proliferation inhibitory effect of postbiotics made from

antioxidizable probiotics against HT-29 cell

Yeeun Kim², Keunho Ji¹˙*

¹Basic Science Research Institute, Pukyong National University, Busan 48513, Korea, ²Microbiology, Pukyong

National University, Busan 48513, Korea

In this study, strains of 8 probiotics were used: Lactobacillus sp. (La1, La2, La3, La4), Leuconostoc sp. (Le1, Le2, Le3,
Le4). They have antioxidant effects. For postbiotics production, sonication cell death method, cell lysate method

and cell free supernatant (CFS) were used. The antioxidant effect is confirmed through DPPH free radical scavenging

and total polyphenol content measurement, and the proliferation inhibitory effect is confirmed on the human colon
cancer cell line (HT-29 cell) through the WST method. When the proliferation inhibitory effect is confirmed, the
number of apoptotic cells of HT-29 cells is measured through flow cytometry. Furthermore, protein expression and

activity are confirmed using caspase, an important factor in the expression of apoptosis, as a primary antibody.The
primary goal of this study is to develop postbiotics that compensate for the disadvantages of existing probiotics,

which are less resistant to the environment. Furthermore, it proves the value of useful substances present in
extracellular and intracellular through the method of manufacturing with postbiotics utilizing both the cells and

metabolites of cells. This study will be a study that proves the usefulness of postbiotics.