Page 51 - M. Immunology

Isoegomaketone Suppressive

M. Immunology.32 DSS-induced Ulcerative Colitis in mice
Ye-Ram Kim, Bomi Nam, Ah-Reum Han, Jin-Baek Kim, Chang Hyun Jin*

BACKGROUND AIM

Inflammatory bowel disease (IBD) has been considered as health Inflammatory bowel disease (IBD) has been considered as
problem worldwide. Previously, our group revealed that isoegomaketone severe health problem. However, the effective cure for IBD
(IK), an essential oil component of radiation-induced mutant Perilla is still lacking, thus potential therapeutic agents is urgently
frutescens, has anti-inflammatory activity. Previous studies needed. The purpose of this study is to verify the
demonstrated that IK has potent anti-oxidant and anti-inflammatory therapeutic effect of IK on ulcerative colitis.
effects on RAW264.7 cells via HO-1/Nrf2 pathway.

METHODS

RESULTS

Figure 1. Effect of IK on production of LPS- Figure 2. Effect of IK on severity of DSS-induced colitis mice.
induced inflammatory cytokines level in BMDM.
IK – 5mg/kg, RA – 30mg/kg
n=8

BMDMs were pretreated with IK at 5, 10 and 20 μM for
2h and were stimulated with LPS (100ng/ml) for 18h.

▶IK reduced level of inflammatory cytokines, such as TNF, IL-6, IL-12p40, and IL-10, in LPS-stimulated BMDM in dose
dependent manner.
▶ IK suppressed severity of DSS-induced colitis in mice, as assessed by survival rate, body weight, and colonic damage.
CONCLUSION REFERENCES

This study shows that IK inhibited the - Jin et al., Isohomaketone Upregulates Heme Oxygenase-1 in
induction of inflammatory cytokines RAW264.7 Cells via ROS/p38 MAPK/Nrf2 Pathway. 2016.
related inflammatory response in LPS- Biomol Ther.
stimulated primary mouse - Jin et al., Isoegomaketone Inhibits Lipopolysaccharide-
macrophages and ameliorated DSS- Induced Nitric Oxide Production In RAW 264.7 Macrophages
induced colitis in mice model. In through the Heme Oxygenase-1 Induction and Inhibition of the
addition, this study shows that radiation Interferon-β-STAT-1 Pathway. 2010. J.Agric.Food Chem. Contact information
breeding technology can useful tool for -Park et al., Perilla frutescens Extracts Protects against
explore effective components from Dextran Sulfate Sodium-Induced Murin Colitis: NF-κB, STAT3, Advanced Radiation Technology Institute,
natural products. Taken together, we and Nrf2 as Putative Targets. 2017. Front Pharmacol. Korea Atomic Energy Research Institute,
suggest that IK can be novel -Jin et al., Rosmarinic acid suppresses colonic inflammation in
alternative therapy for treatment dextran sulphate sodium (DSS)-induced mice via dual Jeongeup-si, Jeollabuk-do 56212, Korea.
colitis. inhibition of NF-κB and STAT3 activation. 2017. Sci Rep. yrkim327@kaeri.re.kr