Page 50 - M. Immunology
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[M. Immunology-31]
Bee Venom Phospholipase A2 alleviates collagen-induced
polyarthritis by inducing Foxp3+ Treg cell polarization in
mice
Gwang Muk Choi¹, Riwon Hong¹˙², Hyunsu Bae¹˙², Dae Hyun Hahm¹˙³
¹Department of Biomedical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea,
²Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Republic of
Korea, ³Department of Physiology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
In the present study, bee venom phospholipase A2 (bvPLA2), a BV enzyme catalyzing the release of membrane
arachidonic acid, is suggested to be a novel anti-inflammatory BV mediator stimulating the CD25+ Foxp3+ Treg
cell polarization. To verify this hypothesis, anti-arthritic effect of bvPLA2 was evaluated in a mouse model of
rheumatoid arthritis (CIA). An experimental arthritis was induced by 2-week-interval double injections of type II
collagen emulsified in complete (1st) and incomplete Freund’s adjuvant (2nd injection) to the base of the tail. After
2nd injection, bvPLA2 (0.1, 0.5, 1.0 mg/kg) was i.p. injected every two days for 5 weeks. Arthritic behaviors were
significantly alleviated, and histological and micro-CT images of arthritic joints were highly coincident with their
behaviors. Anti-arthritic effects of bvPLA2 became extinct by 0.25 mg/kg anti-CD25 IgG and 10 μg/kg P60, a peptide
inhibitor of Treg, as regards arthritic behaviors. Interestingly, it was observed in flow cytometric analysis of major T
cell subsets from spleen that Tregs were completely depleted by the anti-CD25 IgG treatment, not by P60. In
summary, bvPLA2 have a significant anti-inflammatory and anti-arthritic activity in the CIA mouse model through
the modulation of development and function of Treg.
