Page 34 - M. Immunology

[M. Immunology-23]

Pig tissue factor pathway inhibitor α has better ability of

inhibiting blood coagulation caused by pig tissue factor than

human counterpart

Chang Hee Lee¹, Taehoon Chun¹˙*

¹Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, Korea

Xenotransplantation is a good solution for the patients who meet with the shortage of volunteer’s organs. Xenograft
rejection caused by pig tissue factor (TF) is one of most severe problem for xenotransplantation. This rejection
induces severe inflammation with complement activation and infiltration of neutrophils. In this study, we invented

fusion protein with the constant region of immunoglobulin and tissue factor of human or pig (TFPI-Ig) to isolate
TFPI. PK15 cell lysate were used for analyzing TF activity downregulating ability of TFPI. Our results revealed that

pig TFPI has better therapeutic effects than human one. However, both TFPIs have cleavable site by some enzymes
in human blood. This study suggests a good possibility to block IBMIR caused in pig to human xenotransplantation

with pig TFPI-Ig.