Page 35 - M. Immunology

Lactobacillus plantarum CJLP133 and CJLP243 alleviates the allergic
rhinitis in BP-inhaled mouse model
Hyeon jeong Lee, Taehoon Chun
Department of Biotechnology, College of Life Sciences and Biotechnology,
Korea University, Seoul, 02841, Republic of Korea

BACKGROUND AIM
Birch pollen (BP)-induced allergic rhinitis is mediated by BP specific IgE-primed In this study, we tested whether CJLP133 and CJLP243 could modulate the
inflammation in the nasal and is classified as a type I airway hypersensitivity. In immune response in an animal model of BP induced allergic rhinitis in mice.
particular, eotaxin, IL-4, IL-5 and IL-13 mediate recruitment and activation of The experimental results demonstrated that oral administration of CJLP133 or
inflammatory cells in lesions. Lactobacillus plantarum CJLP133 and CJLP243 were CJLP243 can induce Th1 responses while suppressing Th2 responses,
originally isolated from Kimchi, and immune cells stimulated with them produced thereby ameliorating major symptoms of BP induced allergic rhinitis in a
high levels of a Th1-type cytokine. Previous study indicated that consumption of mouse model.
CJLP243 improved the growth performance of weaning pigs during acute infection
of pathogenic enteric bacteria by enhancing Th1-type immune response.
METHOD
Allergic rhinitis was induced in BALB/c mice by sensitization to BP allergen, as previously described. Briefly, mice were sensitized by intraperitoneal injection of 10 μg
Bet v1 mixed with 2 mg Alum on days 0 and 7. Fourteen days later, the mice were challenged with 8 mg of BP extract in 30 ml of PBS via inhalation using an
ultrasonic nebulizer (NE‐U12) (Omron, Kyoto, Japan) for three consecutive days. All experimental mice were killed 1 day after the final BP extract challenge .
Next, 35 BALB/c mice were randomly separated into five groups. Two groups were orally administered different strains of L. plantarum (one strain per group). Each
group received 400 μl of PBS containing L. plantarum CJLP133 or CJLP243 (1 × 1010 CFU per mouse) daily starting 1 week before the sensitization until the mice
were killed
RESULTS
Figure 1 Figure2

Figure 1 Experimental design and effects of Lactobacillus plantarum CJLP133
and CJLP243 on AHR and lesions associated with BP-induced allergic rhinitis
in mice. Figure 2 Oral administration of Lactobacillus plantarum CJLP133 and
(a) After H&E staining, the number of infiltrated cells in the lamina propria of the CJLP243 reduces infiltration of inflammatory cells and modulates
cytokine production in the BAL fluid of mice after induction of BP-
nasal mucosa was counted in the samples (2500 µm2) under a microscope at x200
magnification. The images shown in the left panels are representative of each group induced allergic rhinitis.
of mice. Data shown in the right panels are the mean numbers of infiltrated cells. (b) (a) Infiltration of inflammatory cells in the BAL fluid from each group of
Histological evaluation of lung tissues was performed under a microscope at 9100 experimental mice. Red arrows indicate PMNs. (b) The proportion of cells in
magnification. The results shown in the left panels are representative of each group the BAL fluid from each group of experimental mice was assessed using Diff
of mice. Data shown in the right panels are the average of the peribronchial and Quik staining. Mouse groups: (□) Noninduction, (▤) Vehicle, (▨) CJLP133,
perivascular inflammation scores. Noninduction, non-BP-treated mice; vehicle, PBS (▩) CJLP243, (■) dexamethasone. (c) ELISAs were performed to detect IFN-
(vehicle)-treated mice; CJLP133, mice orally administered CJLP133 in PBS; c, IL-4, IL-5, IL-13, and eotaxin in the BAL fluid. The data shown are means
CJLP243, mice orally administered CJLP243 in PBS; dexamethasone, mice orally ±SEM (n = 7). Significant differences compared with the vehicle are
administered dexamethasone in PBS. The data shown are means ±SEM (n = 7). represented as *P < 005 or **P < 001.
Significant differences compared with the vehicle are represented as *P < 005 or **P
< 001.
Figure 3 Figure 3 Oral administration of Lactobacillus plantarum CJLP133
and CJLP243 decreases the total number of immune cells and
modulates cytokine production in the draining lymph nodes of
mice after induction of BP-induced allergic rhinitis.
(a) The size of the mediastinal lymph nodes in each group of
experimental mice was compared. (b) Total cell numbers in the
mediastinal lymph nodes from each group of experimental mice. (c)
Single cell suspensions from each draining lymph node (1x106 cells per
well) were stimulated with BP extract (100 mg ml-1). Two days after
stimulation, production of IFN-c, IL-4, IL-5 and IL-13 in the culture
supernatants was measured by ELISA..

CONCLUSION REFERENCES CONTACT INFORMATION
The data clearly showed that oral Mastrorilli, C., Posa, D., Cipriani, F. and Caffarelli, C. (2016) guswjdlee@gmail.com
administration of CJLP133 or CJLP243 Asthma and allergic rhinitis in childhood: what’s new. Pediatr (Hyeon jeong Lee)
can alleviate the symptom of BP Allergy Immunol 27, 795–803.
induced allergic rhinitis in a mouse
model by rebalancing the Th1 and Th2 Lee, J., Yun, H.S., Cho, K.W., Oh, S., Kim, S.H., Chun, T.,
immune responses via increasing the Kim, B. and Whang, K.Y. (2011) Evaluation of probiotic
Th1 response. characteristics of newly isolated Lactobacillus spp.: immune
modulation and longevity. Int J Food Microbiol 148, 80–86.