Page 58 - I. Chemical biology and drug discovery

[I. Chemical biology and drug discovery-32]

Suppression of angiogenesis by PD in human umbilical vein

endothelial cells

Hyun-Ji Park¹, Shin-Hyung Park¹˙*

¹Department of Pathology, College of Korean Medicine, Dongeui University, Busan 47227, Korea

PD is a traditional medicinal herb used in Korea for cure of lung-related symptoms. This study investigated the anti-
angiogenic effects of PD. Exposure of human umbilical vein endothelial cells (HUVECs) to ethanol extract of PD
(EPD) significantly decreased the tube formation in a concentration-dependent manner. In addition, results from the

transwell assay showed that EPD markedly suppressed the cell migration of HUVECs when the conditioned medium

(CM) from H1299 human lung cancer cells was used as a chemoattractant. These results indicate that EPD inhibited
the active migration of endothelial cells toward cancer cells. The activation of vascular endothelial growth factor
receptor 2 (VEGFR2), a prominent mediator of angiogenesis, and its downstreams, including Akt, Src and ERK, was

abrogated by EPD. Interestingly, CM from EPD-treated H1299 cells attenuated the cancer cell-induced chemotaxis
of HUVECs. The mRNA levels of VEGF, fibroblast growth factor (FGF)-2, and angiopoietin-1 were down-regulated in

H1299 cells by EPD, suggesting that these factors are potent chemoattractants for endothelial cells. Taken together,
our results demonstrate that EPD possesses anti-angiogenic activities by inhibition of VEGFR2 signaling pathway

(NRF-2019R1F1A1059588).