Page 56 - I. Chemical biology and drug discovery

[I. Chemical biology and drug discovery-31]

Hexane fraction of Adenophora triphylla var. japonica

triggers apoptosis by inhibiting STAT3 and Src in human

non-small cell lung cancer cells

Hyun-Ji Park¹, Shin-Hyung Park¹˙*

¹Department of Pathology, College of Korean Medicine, Dongeui University, Busan 47227, Korea

Adenophora triphylla var. japonica (AT) has been used in traditional Oriental medicine for controlling various airway
inflammatory diseases. In the current study, we investigated the anti-cancer activities of different fractions of AT in
non-small cell lung cancer (NSCLC) cells. Among three kinds of fractions of AT, haxane fraction of AT (HAT)

significantly reduced the cell viability in human NSCLC cells. HAT also reduced the colony formation in H1299 and
H460 cells. To examine whether these phenomena are related with apoptosis, we next performed DAPI staining and

flow cytometry. HAT increased chromatin condensation, sub-G1 DNA content as well as annexin V-positive cells.
We also observed that HAT increased the cleavage of caspase-3, -8, -9, and PARP, indicating that HSE induced

apoptosis in NSCLC cells. Notably, the phosphorylation of signal transducer and activator of transcription 3 (STAT3)
and Src was significantly decreased by HAT. Transfection of constitutively active STAT3 or Src reversed the anti-

proliferative effect of HAT in H1299 cells. Taken together, our findings clearly suggest that inhibition of STAT3 and
Src mediated HAT-induced apoptosis in NSCLC cells (NRF-2016R1C1B2015076, NRF-2019R1F1A1059588).