Page 62 - I. Chemical biology and drug discovery

[I. Chemical biology and drug discovery-34]

DD induced apoptosis in human non-small cell lung cancer

cells with different EGFR mutation status

Hyun-Ji Park¹, Shin-Hyung Park¹˙*

¹Department of Pathology, College of Korean Medicine, Dongeui University, Busan 47227, Korea

DD is a marine brown alga collected from Jeju island. The aim of the current study is to examine the anticancer
effects of DD in human non-small cell lung cancer (NSCLC) cells. We used three kinds of NSCLC cell lines including
H1299, an epidermal growth factor receptor (EGFR)-wildtype, PC9, an EGFR-mutant (delE746-A750) and EGFR

tyrosine kinase inhibitor (TKI)-sensitive, and H1975, an EGFR-mutant (L858R/T790M) and EGFR TKI-resistant cell lines.

The ethanol extract of DD (EDD) decreased the cell viability in these cell lines at 25-100 µg/ml. In addition, EDD
stimulated chromatin condensation and increased the cleavages of PARP and caspase-3. The enhanced proportion
of sub-G1 phase cells and the annexin V-positive cells following EDD treatment collectively demonstrated that EDD

triggered apoptosis in NSCLC cells regardless of EGFR mutation status. In addition, phosphorylation of signal
transducer and activator of transcription 3 (STAT3), Src, AKT and ERK, key regulators of cell growth, was rapidly

decreased by EDD. Taken together, we demonstrate that EDD exhibits anticancer effects in NSCLC cells. We propose
that DD could be a novel candidate for managing EGFR-TKIs resistant as well as EGFR-TKI naive NSCLC patients

(NRF-2019R1F1A1059588).