Page 162 - D. Cancer biology

[D. Cancer biology-104]

Identificationof a novel compound that inhibits cancer

metastasis through regulation of the Akt signaling pathway

and Twist1

Jain Ha¹, Ba Reum Kim¹, Sayeon Cho¹, Sewoong Lee¹, Jiyoung Park¹, Eunjeong Kang¹, Jihye Seo¹,

Haelim Yoon¹, Junho Lee¹

¹Laboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, seoul
06974, Republic of Korea

The process of spreading cancer cells to other body parts is called metastasis, which is the major cause of cancer
deaths. Since it is difficult to remove cancer cells once they spread out, suppressing cancer metastasis is a major

challenge in conquering cancer. In this study, we investigated a chemical that had anti-cancer effects as a way to
prevent the metastatic activity of human liver cancer cell line, SK-Hep1. The compound 3-acetyl-5,8-dichloro-2-

((2,4-dichlorophenyl)amino)quinolin-4(1H)-one (ADQ) showed significant anti-cancer effects, including suppression
of wound healing and cellular invasion. Furthermore, cellular growth and multicellular tumor spheroid

survival/invasion of SK-Hep1 cells were significantly inhibited by ADQ. The anti-metastatic effect and anti-
proliferative effect of ADQ were mediated by inhibition of the AKT signaling pathway and downregulation of Twist1

protein levels. Therefore, these findings suggest that the ADQ may be an efficient candidate for cancer drug
development.