Page 160 - D. Cancer biology

[D. Cancer biology-102]

Comparison of sensors to interrogate biomarkers in

Hepatocellular Carcinoma

Dongwoon Han¹, Si-Cho Kim², Young-joon Kim³, Jeongsil Ha¹˙*

¹Integrative Bioscience & Biotechnology, Sejong University, Seoul 05006, Korea, ²Integrated Omics for Biomedical

Science, Yonsei University, Seoul 03722, Korea, ³Biochemistry, Yonsei University, Seoul 03722, Korea

Hepatocellular carcinoma is one of the most prevalent cancers in Asia but lacks efficient therapeutic treatment

except for liver transplantation. Tumor heterogeneity is one of the obstacles to efficient diagnosis and treatment of
cancer. In many cancers, tumor-associated methylations is not only important for early cancer detection but also
for therapeutics. As each patient respond differently to the therapy, specific prognosis subtype distinctions would

be clinically informative. We identified several DNA methylation biomarkers that are associated with prognosis in
hepatocellular carcinoma patients. To validate their clinical utility and reach clinical settings, we designed and tested

various sensing systems that can discriminate different status of DNA methylation in those biomarkers. We tested
MSP, molecular beacon probing, iDDS and HRM to find most effective sensing system. We will present current status

of development and hope our result can contribute to precision medicine. *Corresponding author