Page 138 - D. Cancer biology

[D. Cancer biology-87]

Extracellular microenvironment change through B16F10

melanoma cancer cell-derived proteins induces properties of

cancer stem-like cells in NIH3T3 cells

Hyeongrok Choi¹, Jin Woong Chung¹˙*

¹Biological science, Dong-A university, Busan 49315, Republic of Korea

Cancer stem cells can cause solid tumors and also have properties of self-renewal and differentiation. And CSC can
have the ability to lead to drug resistance and relapse in many tumors. However, there is a lack of understanding
of the origins of the CSC and research on specific targeted therapies. Therefore, it is necessary to establish a cell

line, such as a cancer stem, in order to develop various studies. In this study, we used the B16F10 melanoma cancer
cell-derived protein lysates to induce colony formation that characterizes CSC from NIH3T3 cells. Induced-colonies

showed self-renewal and differentiation capacities through anchorage-independent culture and re-attached culture.
The induced-colonies expressed not only high level drug efflux capacity but also various CSCs markers. In addition,

the induced-colonies clearly formed solid tumor in vivo, clearly demonstrating the tumorigenic ability. These results
show that protein lysates of cancer cells could transform normal cells into CSCs and increase the expression of CSCs

markers. This study argues the tremendous importance of the extracellular microenvironmental effect on the
generation of CSCs. It provides a simple experimental method for deriving CSCs that could be based on the

development of targeted therapy techniques.