Page 143 - D. Cancer biology

Inhibitory Effect of Globefish Homogenate on the Growth of
Human Colorectal Cancer Cell Lines

Junghoon Kim, and Jungho Kim

ABSTRACT

Cancer is one of the most serious public health problems in Korea and worldwide, and has a major impact on society. Despite significant advances in traditional anti-cancer therapies, such as
chemotherapy, radiotherapy, and surgery, it remains extremely difficult to cure and prevent cancer. Consequently, novel anti-cancer therapies must be developed. We previously reported that
Globefish homogenate suppresses the growth of Caco-2 human colorectal cancer cells. To build upon this previous observation, we investigated the cytotoxic effects of Globefish homogenate on
DLD-1, HCT116, and SW480 human colorectal cancer cells by counting cells and examining morphological changes underneath an inverted microscope. Treatment with Globefish homogenate
markedly inhibited the growth of cultured DLD-1, HCT116, and SW480 cells in a dose-dependent manner. The sensitivities of these cells to Globefish homogenate were determined by calculating
the lethal concentrations. The 50% inhibitory concentrations of Globefish homogenate were 111, 95, and 121 ug/ml in DLD-1, HCT116, and SW480 cells, respectively. These results indicate that
Globefish homogenate contains a bioactive material(s) that inhibits the growth of human colorectal cancer cells
Effects of Globefish homogenate on growth of SW480 human colorectal cancer cell
RESULTS

Cytotoxic effects of Globefish homogenate on DLD-1, HCT116, and SW480
human colorectal cancer cells

Fig. 4. Effects of Globefish homogenate on growth of SW480 human colorectal cancer cells (A) Representative
images of SW480 Growth without or with 1% Globe Homogenate treatment for 6days, Phase contrast, X100.
Human colorectal cancer cell SW480 was plated into 12-well plates at a density of 10000 cells/well. (B) The total
number of SW480 human colorectal cancer cells without or with 1% Globe homogenate was calculated every
other day for 8days.
IC50 of Globefish homogenate on DLD1 human colorectal cancer cell proliferation
Fig. 1. Inhibition of Colony forming effect in human colorectal cancer cell lines with Globefish Homogenate. Crystal
violet staining of human colorectal cancer cell lines DLD1, HCT116, SW480 at 4and 8day without or with 1%
Homogenate. (B) Colony formation was reduced with 1% Globe Homogenate, compared to Control at 6day. ** P
< 0.01.
Effects of Globefish homogenate on growth of DLD1 human colorectal cancer cell

Fig. 5. IC50 of Globefish homogenate on DLD1 human colorectal cancer cell proliferation. (A)
Representative images of DLD1 human colorectal cancer cells with 0%, 0.001%, 0.01%, 0.1%, 1% Globe
Homogenate at 6days, Phase contrast, X100. (B) IC50 values of Globe Homogenate in DLD1 human
colorectal cancer cells. All values are expressed as means ± SD of 3 independent experiments, each
done in duplicates.
IC50 of Globefish homogenate on HCT116 human colorectal cancer cell proliferation

Fig. 2. Effects of Globefish homogenate on growth of DLD1 human colorectal cancer cells (A) Representative
images of DLD1 Growth without or with 1% Globe Homogenate treatment for 6days, Phase contrast, X100.
Human colorectal cancer cell DLD1 was plated into 12-well plates at a density of 10000 cells/well. (B) The total
number of DLD1 human colorectal cancer cells without or with 1% Globe homogenate was calculated every other
day for 8days.
Effects of Globefish homogenate on growth of HCT116 human colorectal cancer cell
Fig. 6. IC50 of Globefish homogenate on HCT116 human colorectal cancer cell proliferation. (A)
Representative images of HCT116 human colorectal cancer cells with 0%, 0.001%, 0.01%, 0.1%, 1%
Globe Homogenate at 6days, Phase contrast, X100. (B) IC50 values of Globe Homogenate in HCT116
human colorectal cancer cells. All values are expressed as means ± SD of 3 independent experiments,
each done in duplicates.
IC50 of Globefish homogenate on SW480 human colorectal cancer cell proliferation

Fig. 3. Effects of Globefish homogenate on growth of HCT116 human colorectal cancer cells (A) Representative
images of HCT116 Growth without or with 1% Globe Homogenate treatment for 6days, Phase contrast, X100.
Human colorectal cancer cell HCT116 was plated into 12-well plates at a density of 10000 cells/well. (B) The total
number of HCT116 human colorectal cancer cells without or with 1% Globe homogenate was calculated every
other day for 8days. Fig. 7. IC50 of Globefish homogenate on SW480 human colorectal cancer cell proliferation. (A)
Representative images of SW480 human colorectal cancer cells with 0%, 0.001%, 0.01%, 0.1%, 1% Globe
Homogenate at 6days, Phase contrast, X100. (B) IC50 values of Globe Homogenate in SW480 human
CONCLUSION colorectal cancer cells. All values are expressed as means ± SD of 3 independent experiments, each done in
duplicates.
.
These results indicate that Globefish homogenate contains a bioactive material(s) that inhibits the growth of human colorectal cancer cells
REFERENCES
Kim, J., Chung, G. & Kim, J. (2017) Inhibitory Effect of Globefish Homogenate on the Growth of Caco-2 Human Colorectal Cancer Cells, KSBB Journal. 32, 212-217
Contact information

Laboratory of Molecular and Cellular Biology, Department of Life Science, Sogang University, Seoul 40107, Korea
E-mail : jkim@sogang.ac.kr, Phone: 82-2-715-8461