Page 146 - D. Cancer biology

[D. Cancer biology-95]

Suppression of MCF-7 Human Breast Cancer Cell Proliferation

by Globefish Homogenate through Downregulation of AKT

Activation

Jung hoon Kim¹, Jung ho Kim¹˙*

¹Laboratory of Molecular and Cellular Biology, Department of Life Science, Sogang University, Seoul 04107, Korea

Breast cancer is one of the most aggressive cancers in women worldwide, including in South Korea. Although
treatment of this cancer has improved significantly, it is still the leading cause of cancer-related death in women.
Previously, we reported that Globefish homogenate suppresses growth of human colorectal cancer cells. To extend

the applications of Globefish homogenate, we investigated its cytotoxic effects on human breast cancer cells.
Addition of Globefish homogenate markedly suppressed the growth of MCF-7 cells in a dose-dependent manner.

The sensitivity of the cells to Globefish homogenate was determined by calculating the 50% inhibitory concentration
(IC50); in this case, the IC50 was 210g/ml. Furthermore, the cytotoxic role of Globefish homogenate was confirmed

by investigating major signaling pathways. Significant downregulation of Cyclin D1 expression, along with phospho-
Akt and total Akt levels, was observed in MCF-7 cells treated with Globefish homogenate. This study demonstrates

that treatment with Globefish homogenate inhibits proliferation of MCF-7 human breast cancer cells by
downregulating expression of phosphor-Akt, total Akt, and Cyclin D1 proteins.