Page 136 - D. Cancer biology

[D. Cancer biology-86]

Study of apoptosis in human lung cancer cells by plasma

activated medium

Ara Jo¹, Hea Min Joh², Tae Hun Chung², Jin Woong Chung¹˙*

¹Biological Sciences, Dong-A University, Busan 49315, Republic of korea, ²Materials Physics, Dong-A University,

Busan 49315, Republic of korea

Cold atmospheric plasma (CAP) can be treated in cells by direct and indirect treatment. There are many reports of
cell death pathways induced by CAP, but there is a lack of fundamental research on how CAP can kill cancer cells.

In this study, we studied how PAM produced by CAP causes cancer cell death. To confirm this, the difference in the

expression of NO₂ and H₂O₂ in PAM cultured with cancer cells was measured, and intracellular ROS changes were
observed using flow cytometry. The amount of various reactive species in PAM generated by a 2.45 GHz microwave
excited atmospheric plasma jet (ME-APPJ) was estimated using colorimetric methods. There was little change in

NO₂ in PAM cultured with human lung cancer cells (A549 cells), but the H₂O₂ concentration gradually decreased
after 30 minutes of treatment with the cells. In contrast, intracellular ROS in A549 cells increased rapidly from 2

hours. On the other hand, little change was found in normal cells. PAM had a significant cytotoxic effect on A549
cells but had little effect on normal cell viability. In addition, it was confirmed that apoptosis of A549 cells by PAM

was increased by using cell cycle analysis. These results suggest that H₂O₂ plays an important role in inducing
cancer cell apoptosis among ROS produced by PAM.