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[A. Biochemistry/Molecular Biology] A-49

Inhibition of histone deacetylase 1/2 enhanced NK cell

mediated anticacenr immunity through induction of NKG2D

ligands

Haeryung Cho¹, Eun Jung YUN¹, Jaeho Bae¹*

¹Convergence science, Pusan National University, Yangsan ks011, Korea

Histone acetylation regulates various ligand and genes expressions with epigenetic mechanisms. NKG2D Receptors
of immune cells are activated by natural killer group 2 (NKG2D) ligands. NKG2D ligand expression is upregulate as

histone deacetylases (HDAC) inhibitors. We predicted that HDAC genes to affect the expression of NKG2D ligands
and observed NKG2D ligands and major histocompatibility complexes (MHC) expressions in cancer cells treated

with HDAC1, HDAC2 inhibitors and specific small interfering RNA (SIRNA). Furthermore, treatment with selective
HDAC1/2 inhibitors was found to induce NKG2D ligand expression in transcription and proteomic. Cancer cells

susceptibility to NK cells was also increased. The increase in NKG2D ligand expression was also seen when HDAC1
or HDAC2 were silenced. These studies show that inhibiting HDAC1 and HDAC2 can induce expression of NKG2D

ligand and NK cell-mediated anticancer immunity improvement.