Page 94 - ebook

[A. Biochemistry/Molecular Biology] A-47

Digging into N-glycosylation in Human Brain and

Cerebrospinal Fluid

Sol kim¹,², Dea Sik Cho¹,², Heejin Jeong¹,², Myung Jin Oh¹,², Hyun Joo An¹,²*

¹Asia Glycomics Reference Site, Chungnam National University, Chungnam National University, Daejeon 34134,

Korea, ²Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134,

Korea

Glycosylation is highly implicated with brain development and functions. Changes in glycosylation have recently
been reported in Alzheimer's disease and intractable epilepsy. However, the study of human brain glycome are

relatively poorly understood compared to other mammalian due to the limited availability of samples and the lack
of analytical tools. Cerebrospinal fluid (CSF), a body fluid that interact directly with the extracellular fluid of the

central nervous system, serves as a source for the neurological study. However, a systematic analysis for correlation
of glycosylation in the CSF and brain regions has not yet been performed. Here, we explored the association of

their glycosylation using LC/MS-based glycomics. A total of 87 and 68 glycans were identified in human brain and
CSF, respectively. Neutral-glycans were dominantly expressed over 70% in both in brain and CSF. Fucosylated-

glycan(Hex3HexNAc5Fuc1) and high-mannose glycan(Man5), known as the most abundant N-glycan in brain tissue,
were identified as major components in CSF. Interestingly, complex type glycans terminal sialic acid epitope were

overexpressed in CSF than in tissues. We will conduct further studies to determine the glycosylation linkage between
CSF and brain regions to explore the use of CSF as a tissue alternative in brain disease diagnosis and biomarker
discovery.