Page 66 - M. Immunology

[M. Immunology-39]

Anti-allergic effect of Catalpa ovata extract on mast cell-

mediated allergic model

Jae Yeon Lee¹˙#, Kitae Park¹, Min Hee Hwang¹, Hye Jin Ko¹, Eun Kyung Ahn¹, Yoojin Han¹, Young Rak Cho¹˙*

¹Medicinal Evaluation Team, Gyeonggido Business&Science Accelerator, Suwon 16229, Republic of korea

Recent studies have shown that mast cell-mediated allergic disorder associated with many diseases such asthma,
atopic dermatitis and allergic rhinitis. The finding of drugs for the treatment of allergic disease is an important
study. In this study, we investigated the effect of Catalpa ovata extract (COE) on the Immunoglobulin E (IgE)-

mediated allergic response in vitro and in vivo. COE significantly inhibited the release of β-hexosaminidase in RBL-

2H3 cells with IC50 value of 94.24 μg/ml, and suppressed the expression of inflammatory chemokines such as
macrophage-derived chemokine (MDC) and thymus and activation regulated chemokine (TARC) in human
keratinocyte (HaCaT) cells. In vivo model, COE significantly decreased IgE-mediated passive cutaneous anaphylaxis

(PCA) reaction and inhibited compound 48/80 induced systemic reactions. It also reduced serum histamine release
in a dose dependent manner in mice. In conclusion, our findings provide that COE inhibits mast cell-derived allergic

response through the downregulation of inflammatory mediators.