Page 64 - M. Immunology
P. 64
[M. Immunology-38]
Enhanced production of IL-23 from dendritic cells by dust
mite allergen Der p upon co-culturing lung epithelial cells
Min-Sook Ryu¹, Jung-In Shin²˙³, Ji-Hyeon Lee²˙³, Perry Ayn Mayson A. Maza²˙³, Min-Ho Choi², Jong-Young Kwak⁴˙⁵˙⁶
¹Department of Allergy, Ajou University School of Medicine, Suwon 16499, South Korea, ²Department of
Pharmacology, School of Medicine, Suwon 16499, South Korea, ³Department of Biomedical Sciences, Graduate
school, Ajou University, Suwon 16499, South Korea, ⁴D Immune System Imaging Core Center, Ajou University,
Suwon 16499, South Korea, ⁵Immune Network Pioneer Researcg Center, Ajou University, Suwon 16499, South
Korea, ⁶Department of Biofabrication Engineering, Nanofaentech Inc. , Kimhae 50969, South Korea
Dendritic cells(DCs)-mediated IL-23 secretion has been considered to be modulated by diverse biological processes.
We investigated whether dust mite allergen(Der p) regulates production of IL-23 in co-culturing of DCs and lung
epithelial cells. Poly(vinyl alcohol)(PVA) and Poly(e-caprolactone)(PCL) nanofibrous membranes were generated via
electrospinning of PVA and PCL in water and chloroform solution, respectively. We assembled a two-layer co-culture
system using PVA and PCL membrane for lung epithelial MLE-12 cell growth in the upper PVA membrane layer
with co-cultured bone marrow-derived DCs (BMDCs) in the lower PCL membrane layer. MLE-12 cells were cultured
for 3 days to form tight junctions and DCs were cultured for 4 h for membrane adherence. After 1 day of treatment
with Der p, BMDCs up-regulated expression of MHC-II and CD86 and secreted IL-23 and pro-inflammatory cytokines
including TNF-alpha and IL-1beta. Secretion of IL-23 but not TNF-alpha and IL-1beta by Der p-treated DCs was
enhanced through co-culturing of MLE-12 cells. The secretion of IL-23 was further enhanced by co-culturing of T
cell with DCs and MLE-12 in the presence of Der p. Thus, IL-23 secretion by DCs is enhanced by dust mite allergen-
-stimulated lung epithelial cells and further amplified by T cells.
