Page 62 - M. Immunology

[M. Immunology-37]

Inotodiol, a novel lanostane triterpenoid from Chaga

mushroom Inonotus Obliquus induces characteristic

maturation of dendritic cells

Perry Ayn Mayson A. Maza¹˙², Ji-Hyeon Lee¹˙², Min-Sook Ryu³, Ludmila P. Ponomarenko⁴, Valentin A. Stonik⁴,

Jong-Young Kwak¹˙²˙³˙*

¹Department of Pharmacology, School of Medicine, Ajou University , Suwon 16499, South Korea, ²Department of

Biomedical Sciences, Graduate school, Ajou University, Suwon 16499, South Korea, ³Department of Allergy, Ajou
University School of Medicine, Suwon 16499, South Korea, ⁴Department of Bioorganic Chemistry, Far Eastern
Branch of the Russian Academy of Sciences, Russian Federation 690022, Russia

Dendritic cells(DCs) have the ability to stimulate naïve T cells that coordinate subsequent adaptive response towards
inflammatory response or tolerance depending on the DC differentiation level. Inotodiol, a lanostane triterpenoid

from Inonotus obliquus (wild Chaga mushroom) is as a natural compound. In this study, we investigated whether
inotodiol promotes maturation of bone-marrow-derived DC(BMDC) and induces T cell proliferation. Inotodiol

increased the expression of surface maturation markers, including MHC-II, CD86, and CD40 on BMDCs with almost
no production of various cytokines, including TNF-α and IL-12p40 from the BMDCs. OT-I T cells primed by OVA-

pulsed inotodiol-treated BMDCs proliferated and produced IL-2 with other cytokines, including IL-12p40 and INF-
γ. Specific inhibitor of PI3K abrogated the effects of inotodiol on upregulation of CD86 and MHC-II, whereas inhibitor

of NF-κB inhibited their expression both by LPS an inotodiol. SB216763, specific inhibitor of GSK-3β alone up-
regulated CD86 expression and augmented the upregulation of CD86 expression by inotodiol in BMDCs. These
results suggest that inotodiol induces DCs a characteristic type of maturation through PI3K activation in

independence of NF-κB and inotodiol-treated DCs enhance T cell production of IL-2.