Page 74 - D. Cancer biology

[D. Cancer biology-51]

Targeting Mitochondrial Fission Factor Triggers Immunogenic

Cell Death

Yu Geon Lee¹, Nuri Lim¹, Kyeong Jin Shin¹, Young Chan Chae¹˙*

¹School of Life Sciences, Ulsan National University of Science and Technology (UNIST), Ulsan 44919, Republic of

Korea

Mitochondrial fission factor (MFF) is a molecular that control mitochondrial size and shape as well as mitochondrial
dynamics that is upregulated in most cancer cells. We developed MFF-based cell permeable peptide (MFF peptide),

which could rapidly rupture mitochondrial integrity and induce cell death against various tumor type. In this study,

we have investigated further antitumor activity of MFF peptide. Our results have revealed that treatment of MFF
peptide acutely induces mitochondrial depolarization and cell death in murine tumor cells including B16F10 and
CT26. In addition, cell death by MFF peptide exhibits the hallmarks of immunogenic cell death including secretion

of high mobility group box1 (HMGB1) and adenosine triphosphate (ATP), and translocation of calreticulin to the
cells surface. Moreover, mice treated with MFF peptide results in increased immune response in serum and tumor

tissue with tumor size reduction. These finding suggests that therapeutic targeting MFF may provide actionable
strategy to impair cancer development and progression.