Page 152 - D. Cancer biology

[D. Cancer biology-98]

Quercetin and quercitrin from Agrimonia Pilosa Ledeb inhibit

cell migration and invasion of colon cancer cells through JNK

signaling pathway

Nguyet-Tran Trinh¹, Soo-A Kim¹

¹Biochemistry, Dongguk University, College of Oriental Medicine, Gyeongju 38066, Korea

Agrimonia pilosa Ledeb (AP) is a perennial herbaceous plant, which is widely distributed in Asia. The extracts of AP
have shown diverse pharmacological properties, such as anti-microbial, anti-inflammatory, and anti-tumor activity.
In the present study, the anti-tumor activity of AP was evaluated. Methanol extract from root of AP was performed

HPLC and obtained 12 fractions. Among those, fraction #4 showed most potent inhibitory effect on the migration
of colon cancer cells. Using LC-HR MS analysis, quercetin and quercitrin were identified as flavonoids in fraction #4.

All fraction #4, quercetin and quercitrin effectively inhibited cell migration, invasion and colony formation in RKO
cells. While the level of E-cadherin was increased, the levels of N-cadherin and vimentin were decreased by those

agents. Although they all activate p38, JNK, and ERK signaling pathways, only SP600125, an inhibitor of JNK pathway,
inhibited the effect of fraction #4, quercetin and quercitrin on cell migration. Collectively, fraction #4, quercetin and

quercitrin effectively inhibit cell migration, invasion, and colony formation through the JNK signaling pathway. These
results suggest A. pilosa and its containing two flavonoids quercetin and quercitrin as a candidate for anti-tumor

treatment.