Page 40 - ebook

[A. Biochemistry/Molecular Biology] A-20

Monitoring of Aberrant Glycosylation in Brain of Mouse

Models of Depression using nanoLC/MS/MS

Hee Young Jo¹,², Boyoung Lee³, C. Justin Lee³, Hyun Joo An¹,²*

¹Asia Glycomics Reference Site, Chungnam National University, Daejeon 34134, Korea, ²Graduate School of

Analytical Science and Technology, Chungnam National University, Daejeon 34134, Korea, ³Center for Cognition

and Sociality, Institute for Basic Science (IBS), Daejeon 34126, Korea

In brain, glycans play a pivotal role in various functions depending on neural cell interactions such as neurite
outgrowth, synapse formation and maturation. Recent studies indicated that glycan is directly or indirectly related

to brain diseases such as neuropsychiatric disorders and neurodegenerative diseases. Here, we first performed
analysis of glycome expression patterns in depression-specific regions in the mice brain with depression using a

cutting-edge experimental tool based on liquid chromatography-mass spectrometry, with ultimate aim to elucidate
the association between depression and glycosylation. In hippocampus and prefrontal cortex, which are behaviorally

well known as brain regions-specific to depression, we spatially observed an abnormal glycosylation. We assigned
extracted glycans based on glycome database in mammalian brain, and then elucidated quantitative glycosylation

changes in mouse brain with depression using statistical analysis. Interestingly, sialylated and fucosylated complex-
hybrid types glycans were decreased in mice brain in depression compared to control. Notably, glycan expression

patterns more highly changed in prefrontal cortex than hippocampus. Our findings reveal association between
behavioral changes specific to depression and biochemical change. Further exploration of glycoproteins and
glycosyltransferases closely related to aberrant glycosylation in depression may help to understand the role of

glycans in the pathogenesis of depression from a broader perspective.