Page 36 - ebook

[A. Biochemistry/Molecular Biology] A-18

Wnt-EGFR signaling induces tumor development in part

through Phosphofructokinase 1 platelet isoform upregulation

So Mi Jeon¹, Jong-Ho Lee¹,²*

¹Department of Health Sciences, The Graduate School of Dong-A University, Busan 49315, Korea, ²Department of

Biological Sciences, The Graduate School of Dong-A University, Busan 49315, Korea

The activation of Wnt signaling has been detected in various types of human cancer and has been shown to be

associated with cancer development. In the present study, it was revealed that Wnt signaling induced the expression
of phosphofructokinase 1 platelet isoform (PFKP), which has been reported to catalyze a rate-limiting reaction in

glycolysis and is important for the Warburg effect, proliferation, colony formation and cancer cell migration.
Moreover, it was demonstrated that Wnt3A induced PFKP expression in a β-catenin-independent manner, resulting

in increased PFK enzyme activity. Wnt3A-induced epidermal growth factor receptor transactivation activated
PI3K/AKT, which stabilized PFKP through PFKP S386 phosphorylation and subsequent PFKP upregulation. Wnt3A-

induced PFKP S386 phosphorylation increased PFKP expression and promoted the Warburg effect, cell proliferation,
colony formation and the migratory ability of cancer cells. On the whole, the findings of the present study

underscore the potential role of PFKP in Wnt signaling-induced tumor development.