Page 16 - ebook

[A. Biochemistry/Molecular Biology] A-8

The effect of PPAR-γ through the regulation of PTEN

expression in human acute promyelocytic leukemia HL-60

cells

On-Yu Hong¹, Eun-Mi Noh¹, Hye-Yeon Jang¹, Jinny Park², Jong-Suk Kim¹*

¹Biochemistry, Medical Sciences, Chonbuk National University Medical School, Jeonju 54907, Korea, ²Internal

medicine, Gil Medical Center, Gachon University College of Medicine, Incheon 21565, Korea

Cell differentiation in acute promyelocytic leukemia indicates differentiation syndrome, which is a side effect of

chemotherapy during treatment. This study contributes to establishing an experimental model using peroxisome
proliferator-activated receptors gamma (PPAR-γ) ligands for the prevention of differentiation syndrome. PPAR-γ play

an important role in the differentiation of different cell lines. We demonstrate that PPAR-γ ligands induce neutrophil/
monocytic differentiation in human acute promyelocytic leukemia HL-60 cells. It has been shown that the novel

synthetic triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO) induces differentiation in leukemia
HL-60 cells. We evaluated for the surface antigen expression associated with myeloid maturation, CD11b and CD14

by flow cytometric analysis and RT-PCR. CDDO induced a HL-60 differentiation through the regulation of PTEN
expression. PTEN also has various cellular function such as cell differentiation. PPAR-γ could synergistically up-
regulate PTEN in human leukemia cells and consequently stimulate the differentiation of these cells. We confirm

that suppression of PTEN with the inhibitor (SF1760) caused reduction of differentiated HL-60 cells. Our results

suggest PPAR-γ an important role in controlling the differentiation of leukemia cells via the regulation of PTEN.