Page 124 - ebook

[A. Biochemistry/Molecular Biology] A-62

Secreted matrix metalloproteinase 1 as a marker for

assessement of bone marrow cell migration enhanced by

tonsil-derived mesenchymal stem cells

Hee-Yeon Kim¹, Hee-Soo Yoon¹, Joo-Won Park¹*

¹biochemistry, Ewha medical school, seoul 07804, Korea

Bone marrow transplantation is a procedure to substitute diseased bone marrow with healthy bone marrow stem
cells. Bone marrow transplantation is performed to treat several types of cancers including hematologic malignancies,

and other immune-related diseases in which abnormal bone marrow is the pathologic mechanism. To achieve
optimal bone marrow engraftment, migration of donor bone marrow cells towards the recipient’s bone marrow is

critical. Although the enhanced engraftment of bone marrow cells by co-administration of tonsil-derived
mesenchymal stem cells (TMSCs) has been previously reported, the efficiency can be various depending on TMSC

donors. Conditioned medium from TMSCs enhanced bone marrow cell migration in different degrees depending
on donors. Analyzing transcriptome and proteome profiles of TMSCs from 25 donors revealed higher levels of

matrix metalloproteinase 1 (MMP1) in TMSCs with high efficacy for bone marrow cell migration. MMP1 knockdown
in TMSCs abrogated the supportive efficacy of TMSCs in bone marrow cell migration. This study suggests secreted
MMP1 can be used as a marker to evaluate the efficacy of TMSCs in enhancing bone marrow cell migration.