Page 6 - T. Protein modification and regulation

[T. Protein modification and regulation-3]

Asymmetric methylation of Arginine-617 of Caprin1 regulates

stress granule transcriptome

So Jeong Eom¹, Da Hee Choi¹, Seung Hoi Koo¹

¹Division of life science, Korea University, Seoul 02841, Republic of Korea

Stress granule(SG) is a form of dynamic RNP granules. In mammalian cells, cell cycle associated protein 1(Caprin1),
a phosphoprotein which is highly conserved in vertebrates, plays an important role in forming SGs by oxidative
stress.

Recent articles suggested that dynamic RNA redistribution could occur due to diverse stresses. So we thought that
post-translational modification of Caprin1 RNA binding site may have impact on RNA granule transcriptome.

Here, we found that when Caprin1 R617 mutated, asymmetric methylation of Caprin1 decreased significantly.
Association of Caprin1 with G3BP1 or PRMT1 also decreased.

In this study, we will delineate the impact of caprin1 mutation on SG transcriptome.

Reference

1. Bedford MT, Clarke SG. Protein arginine methylation in mammals: who, what, and why. Mol Cell. 2009;33(1):1-13.
doi:10.1016/j.molcel.2008.12.013

2. Khong A, Matheny T, Jain S, et al. The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in
Stress Granules. Molecular Cell. 2017 Nov;68(4):808-820.e5. DOI: 10.1016/j.molcel.2017.10.015.

3. Namkoong, S., Ho, A., Woo, Y. M., Kwak, H., & Lee, J. H. (2018). Systematic Characterization of Stress-Induced
RNA Granulation. Molecular cell, 70(1), 175–187.e8. https://doi.org/10.1016/j.molcel.2018.02.025