Page 2 - T. Protein modification and regulation
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[T. Protein modification and regulation-1]
Tol2 transposon-based vector platform for the efficient
protein expression
Su Young Hwang¹, Joon Tae Park¹
¹Division of Life Sciences, Incheon National University, Incheon 22012, Korea
The establishment of mammalian cell lines with high productivity is the most important criterion in the field of
recombinant therapeutic protein production. Transposon system has been found to be a reliable tool that enables
the increased transgene integration. In this study, we used Tol2 transposon system which requires a Tol2 transposase
that is needed to insert transposon vector (TP) into the genome. To prevent the risk of genomic instability due to
uncontrolled activity of Tol2 transposase in DNA vectors, we used Tol2 transposase mRNA. We found that the Tol2
transposon system significantly increased transgene expression. To further improve low transfection efficiency due
to large vector size, a mini-transposon vector (mini-TP) consisting of a minimum cis-sequence for Tol2 transposon
was designed. We found that the mini-TP exhibits the same activity as the original TP. Furthermore, inhibition of
DNA methylation prevented gene silencing that may occur in the Tol2 transposon-mediated system. Taken together,
our results are the first report to show that the use of the Tol2 transposon system with Tol2 transposase mRNA
improved protein productivity. Furthermore, the potential use of mini-TP in the biopharmaceutical industry was
evidenced by similar productivity to traditional TP.
