Page 4 - N. Metabolism and metabolic diseases

[N. Metabolism and metabolic diseases-2]

Comprehensive multi-omics analysis reveals aberrant

metabolism of Epstein-Barr-Virus-associated gastric

carcinoma

Jun Yeob Kim¹˙#, Sang Jun Yoon²˙#, Nguyen Phuoc Long², Jung Eun Min², Hyung Min Kim², Jae Hee Yoon¹,

Nguyen Hoang Anh², Myung Chan Park¹, Sung Won Kwon²˙*, Suk Kyeong Lee¹˙*

¹Department of Medical Life Sciences, Department of Biomedicine & Health Sciences, College of Medicin, The

Catholic University of Korea, Seoul 06591, South Korea, ²College of Pharmacy, Seoul National University, Seoul
08826, South Korea

The metabolic landscape of Epstein–Barr-virus-associated gastric cancer (EBVaGC) remains to be elucidated. We

used transcriptomics, metabolomics, and lipidomics to comprehensively investigate aberrant metabolism in EBVaGC.
Specifically, we conducted gene expression analyses using microarray-based data from gastric adenocarcinoma

epithelial cell lines and tissue samples from patients with clinically advanced gastric carcinoma. We also conducted
complementary metabolomics and lipidomics using various mass spectrometry platforms. We found a significant

downregulation of genes related to metabolic pathways, especially the metabolism of amino acids, lipids, and
carbohydrates. The effect of dysregulated metabolic genes was confirmed in a survival analysis of 3951 gastric

cancer patients. 15 metabolisms related to polar metabolites and 15 lipid-associated pathways were involved in
alteration of metabolites by EBV in gastric cancer. In this work, we have described the metabolic landscape of

EBVaGC at the multi-omics level. These findings could help elucidate the mechanism of EBVaGC oncogenesis.