Page 28 - N. Metabolism and metabolic diseases
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[N. Metabolism and metabolic diseases-15]
Carbonic anhydrase regulates anabolic and catabolic factors
in articular chondrocytes
Yunhui Min¹, Dahye Kim², Dinesh Suminda Godagama Gamaarachchige¹, Xiangyu Zhao¹, Mangeun Kim²,
Young-Ok Son¹˙²˙*
¹Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University,
Jeju 63243, Republic of Korea, ²Animal Biotechnology, Faculty of Biotechnology, College of Applied Life Sciences,
Jeju National University, Jeju 63243, Republic of Korea
Osteoarthritis (OA) is caused by imbalance of anabolic and catabolic factors. An articular cartilage is one of the
most avascular tissue and it is relatived with the hypoxic and acidic milieu. Carbonic anhydrases (CA) enzymes are
zinc-containing metalloenzymes that catalyze reversible hydration-dehydration of carbon dioxide and bicarbonate.
Therefore, CA is important for the regulation of acid-base status in the cells. However, a little information is available
about the effects of pH imbalance in cartilage degeneration. Here, we investigated the roles of pH imbalance in
cartilage degeneration in chondrocytes by focusing on the CA enzymes. Microarray analysis revealed that among
16 CA family members, Car9, Car12, and Car13 are specifically upregulated in OA chondrocytes. We further
confirmed the microarray data by the RT-PCR. Moreover, adenovirus-mediated overexpression of Car9, Car12 and
Car13 modulates anabolic or catabolic factors in articular chondrocytes. Adenovirus-mediated overexpression of
Car9, Car12, and Car13 in mouse knee-joint tissue also modulates OA pathogenesis. Taken together, these results
suggested that CA might be new key factors in cartilage degeneration where pH imbalance is an important mediator
in OA pathogenesis.
