Page 24 - N. Metabolism and metabolic diseases

[N. Metabolism and metabolic diseases-12]

Anti-obese mechanisms of anthocyanin-rich black soybean

testa (ABST-3) in the beige-like adipocytes (BLA)

Minjee Lee¹, Juhee Kim², Suyeon Lee², Dajeong Son², Miyoung Park¹, Myoungsook Lee¹˙²˙*

¹Food and Nutrition, Sungshin Women’s University, Seoul 01133, South Korea, ²Research Institute of Obesity

Sciences, Sungshin Women’s University, Seoul 01133, South Korea

There were many studies that the effects of non-shivering thermogenesis and metabolic power of brown
adipocytes(BAT) have been recognized. To find those effects of beige-like adipocytes(BLA) on preventing obesity is

challengeable. Using the extracts of anthocyanin-riched black soybean testa(ABST-3), the purpose of this study was

to confirm energy mechanisms of BLA on preventing obesity. ABST-3 dose-dependently reduced the lipid synthesis
and accumulation in both WAT and BLA. Glycerol contents in the media and phosphorylation of AMPKα as a lipolysis
marker were increased by ABST-3, and the lipolysis effect was significantly higher in BLA than in WAT. The biomarkers

of beiging from white adipocytes, mitochondrial-RFP staining and oxygen consumption to find mitochondria
activation were sharply increased in the adipocytes treated ABST-3 rather than the untreated one. They were also

higher in BLA than in WAT. In conclusion, ABST-3 activated mitochondria and facilitated the formation of BLA from
WAT with mechanism of induction of thermogenesis and energy consumption. With repeated scientific evidences,

the ABST-3 can be recommended as a functional foods that has anti-obesity effects. This work was supported by
the NRF grant(2019R1A2C1008434) and RDA grant(No. PJ015269012020).