Page 8 - M. Immunology

[M. Immunology-5]

The function of INHBB with regard to pregnancy disorder,

preeclampsia in human trophoblast cell line, Swan71 cell

Hee-Na Jeong¹˙#, Eun-Bee Jeong¹˙#, Se-Jin Ahn¹, Ga-Eun Yoo¹, Hae-Ryeong Lim¹˙*, Deug-Chan Lee¹˙*

¹Department of Medical biotechnology, College of Biomedical Science, Kangwon National University, Chuncheon-

si, Gangwon-do 200-701, Republic of Korea

Preeclampsia is a serious pregnancy disorder characterized by gestational hypertension and proteinuria. During the
early stage of pregnancy, trophoblast surrounding the embryo attach to and invade the uterus. Trophoblasts

subsequently perform spiral artery remodeling by interacting with a variety off actors such as hormones, cytokines,

and growth factors. MicroRNAs are small(21-to 23-nucleotide), single-stranded RNA molecules. They bind to
messenger RNA which has complementary sequences and regulate gene expression at the post-transcription level.
In preeclampsia patents over express the miR-210 more than normal pregnancy, we identified that regulated genes

by miR-210 in human first-trimester trophoblast cell line. In present study, we focused on inhibin beta B (INHBB)
which is one of the down-regulated genes by miR-210. We found that INHBB is a direct miR-210 target, down

regulating INHBB mRNA and INHBB protein expression. This miR-210 induced decrease in INHBB expression
suppressed trophoblast cell invasion. Therefore down-regulation of INHBB, a direct miR-210 target, may contribute

to preeclampsia pathogenesis by attenuating trophoblast invasiveness.