Page 12 - M. Immunology

[M. Immunology-8]

Positive effect of Retnla deficiency against

lipopolysaccharide-induced septic shock

Seo Yeon An¹, Kyu Seong Park², Jae-Hoon Choi², Mi-Ran Lee³

¹Department of Medical Science, Chungnam National University, Daejeon 34134, Korea, ²Life Science, College of

Natural Sciences, Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea, ³Biomedical
Laboratory Science, Jungwon University, Chungbuk 28024, Korea

Sepsis is a life-threatening fatal disease and its principal cause is the disparity between pro- and anti- inflammatory

immune responses. Resistin-like molecule alpha (Retnla) regulates the pathogenesis of inflammatory diseases such

as parasitic diseases and asthma. However, the role of Retnla in inflammatory responses against bacterial infection
is unknown. Here we investigated the effects of Retnla on the production of endotoxemia-induced septic responses.
To explore the effects of Retnla on inflammation in vivo, we employed a lipopolysaccharide (LPS)-induced murine

sepsis model and injected LPS (10 mg/kg; Escherichia coli O111:B4) intraperitoneally into mice in the presence or
absence of Retnla. We found that Retnla deficiency attenuated tissue damage of lung and kidney after LPS

stimulation and improved the survival rate. Moreover, Retnla deficiency reversed the reduced cell viability of
RAW264.7 induced by LPS and suppressed the expression levels of IL-1b and iNOS in LPS-activated RAW264.7 cells.

In conclusion, these results show the anti-inflammatory properties of Retnla deficiency on LPS-induced inflammation
and sepsis. Targeting this inflammatory role of Retnla may be a promising area for the development of therapeutic

agents to regulate inflammatory disorders.