Page 17 - M. Immunology

Nuclear Trafficking Inhibition Of SRTFs Protects Lung
In Pneumonitis Animals By Suppressing Cytokine Storm

Gyunam Kim, Dongho Kim, Danbi Lee, Sanghyeon Yu, Misuk Baek, Jieun Kim, Mingu Kang, Hakyoung Park, Jaewook Lee,
Seokwon Lee, Hyemin Yu, Hyewon Lee, Dasom Shin, Bo-ram Kim, Youngsil Choi, and Daewoong Jo
Anti-Cancer Drug Development Team, Cellivery R&D Institute, Cellivery Therapeutics, Inc., Seoul 03929, Korea.
BACKGROUND AIM
Acute respiratory distress syndrome (ARDS) is fatal disease caused Intracellulary delivery of NLS is supposed to suppress expression of
by severe inflammation in lung after infection. Excessive secretion of pro-inflammatory cytokines/chemokines by inhibiting transport of
pro-inflammatory cytokine recruits immune cells and it results SRTFs from cytoplasm into nucleus. iCP-NI regulated expression of
disruption of lung bronchi and alveoli. Excessive secretion of pro- cytokines and chemokine. iCP-NI has potential as a novel medicine
inflammatory cytokines and chemokines accompanied by the to treat the bacterial and viral pneumonitis caused by various
disruption of the bronchi and alveoli which could ultimately develop infections which accompany severe respiratory disorder and death.
into permanent lung impairments such as pulmonary fibrosis.
METHODS

To prevent the unregulated cytokine secretion, called cytokine storm, improved cell-permeable nuclear import inhibitor (iCP-NI) has been
developed by fusing hydrophobic cell-penetrating peptide (CPP) with nuclear localization signal (NLS) and demonstrated its improved
therapeutic applicability.
RESULTS

Figure 1. iCP-NI Inhibits Transport Of NF-κB Figure 4. iCP-NI Suppresses Immunes Cell Homing, Tissue Inflammation &
From Cytoplasm To Nucleus Cytokines In Poly I:C-Induced Pneumonitis Animals

Figure 2. iCP-NI Protects Lung From Pulmonary
Fibrosis In Bleomycin-Induced Fibrosis Animals

Figure 5. iCP-NI Recovered Clinical Symptoms,
Reduced Viral Titer & Fibroflasia In SARS-CoV-2 Infected Monkeys

Figure 3. iCP-NI Protects Apoptosis Of
Immune Cells In Pneumonitis Animals Spleen

CONCLUSION REFERENCES Contact Information
Chung et al. (2020) Science Advances, 6: eaba 1193
iCP-NI suppress expression of pro-inflammatory Minyong Jung
cytokines/chemokines by inhibiting transport of Lim et al. (2013) Clinical Cancer Research, 19: 680-690
New Drug & Business Development
SRTFs from cytoplasm into nucleus. iCP-NI has Lim et al. (2013) Biomaterials, 34: 6261-6271
Cellivery Therapeutics, Inc.
potential as a novel innate immunity-regulating
Lim et al. (2012) Molecular Therapy, 20: 1540-1549
anti-viral / anti-inflammatory immunotherapeutic jungmy@cellivery.com
Jo et al. (2005) Nature Medicine, 11: 892-898
agent for COVID-19 patients. +82-2-3151-8900
Jo et al. (2001) Nature Biotechnology, 19: 929-933