Page 4 - L. Genetics and genomics

[L. Genetics and genomics-7]

Cloning and functional characterization of insect odorant

receptors

Junho Lee¹

¹Biotechnology, Chonnam National University, Gwangju 61186, Korea

The highly specialized olfactory receptor neurons (ORNs) on the antennae of male moths can recognize blends of
several pheromone components. In present study, a total of six candidate pheromone compounds are found and
functionally characterized in the electophyological study. In here, we report on novel candidate pheromone

compounds in the same species. The olfactory receptor is analysed revealed that finding compounds are specifically

affect in on olfactory receptor. In silico study revealed that odorant-gated ion channels comprised of a highly
conserved co-receptor and our chemicals are binding at extra cellular site. Functional analyses on the odorant-
gated ion channels comprised of a highly conserved co-receptor were then performed using the heterologous

expression system of Xenopus oocytes. pheromone components did not respond to any tested pheromone
components and analogs. These results may contribute to clarifying how pheromone detection works in odorant-

gated ion channels comprised of a highly conserved co-receptor.