Page 3 - L. Genetics and genomics

Cloning and functional characterization of insect odorant receptors

1,
Je Jun Oh 1,2 and Jun-Ho Lee *

1 Department of Biotechnology, Chonnam National University, Gwangju, Korea

2 Microzyme corporation, Damyang Samanli 234, Jeonmam, Korea

Summary of the Drosophila Chromosome Screen

ABSTRACT

The highly specialized olfactory receptor neurons (ORNs) on

the antennae of male moths can recognize blends of several

pheromone components. In present study, a total of six

candidate pheromone compounds are found and functionally

characterized in the electophyological study. In here, we report

on novel candidate pheromone compounds in the same species.

The olfactory receptor is analysed revealed that finding

compounds are specifically affect in on olfactory receptor. In

silico study revealed that odorant-gated ion channels comprised

of a highly conserved co-receptor and our chemicals are

binding at extra cellular site. Functional analyses on the

odorant-gated ion channels comprised of a highly conserved

co-receptor were then performed using the heterologous

expression system of Xenopus oocytes. pheromone components

did not respond to any tested pheromone components and

analogs. These results may contribute to clarifying how

pheromone detection works in odorant-gated ion channels

comprised of a highly conserved co-receptor.

Key words: pheromone receptor; pheromone; Xenopus oocytes

MATERIALS AND METHODS

Cloning of Drosophila odorant-gated ion channels

Recording and Data analysis

Two-microelectrode voltage-clamp recording (Oocyte Clamp Adopted from Cell. 2017 Aug 10;170(4):736-747.e9.

(OC-725C), Digidata 1200A)

RESULTS

Figure 1. Gene cloing of Drosophila odorant-gated ion channel (Orco)

SUMMARY AND CONCLUSION

In this study, whole cell voltage clamp recording was

performed with cell expression system of OR65 gene, which

is a subtype of olfactory neuro-receptor isolated from

Drosophila. After the successful expression of this receptor,

microbial culture extract of microorganism, a harmful insect

inducer, was used to investigate whether olfactory receptor

DNA library
activity was regulated. The activity of the receptor was

confirmed in the microbial culture extract.
Library of Drosophila odorant-gated ion channels The

Scripps Research Institute (La Jolla, CA)

Odorant receptor of Apocrypta bakeri, Protein Data Bank (ID A We show that it is possible to identify attractant or repellent

code B0FAQ4, 3.5A resolution) substance using the olfactory receptor activity regulating
system of insects. MZ01 shows the attracting phenomenon by

Figure 2. Drosophila odorant ion channel expression activating insect receptor OR65, The results of the scientific

analysis of the performance of the extracts are presented.