Page 2 - L. Genetics and genomics

[L. Genetics and genomics-5]

Better expression methods of transposon system

YunHaeng lee¹

¹Protein engineering lab, incheon national university, incheon 22012, korea

Among them, the transposon system has been found to be a reliable tool that enables the integration of transgenes.
In this study, we used the SB transposon vector and SB100X transposase vector needed to insert into the genome.
This system showed better protein expression than the conventional vector system. Our study showed that ITR (IR

/ DR) was modified at both ends of the SB transposon to show higher protein expression than the existing SB

transposon system. This was possible because the IR / DR size was small enough and was designed to have a small
impact on the overall vector size. In addition, inhibition of DNA methylation by chemical treatment significantly
improved protein expression by preventing gene silencing that could occur in the SB transposon-mediated system.

(Also, significant differences were found in the amount of transposon vector integration depending on the timing
of methylation inhibitor treatment.) In conclusion, the results of the present invention are the first reports to show

that the use of transposon systems has improved protein productivity in the past. In addition, the use of SB
transposon systems developed in the biopharmaceutical industry will increase production efficiency.