Page 9 - K. Development and regeneration

YAP s ignaling regulations in C VP morphogenesis
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Tae-Young Kim , Ji-Youn Kim , Yam Prasad Ayral , Eui-Seon Lee , Elina Pokharel , Chang-Hyeon An , Hitoshi Yamamoto , Wern-Joo
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Sohn , Jae-Young Kim , Jae-Kwang Jung 1
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1 School of Dentistry, IHBR, Kyungpook National University, Daegu , Korea, Department of Dental Hygiene, Gachon University, Incheon,
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3 Department of Histology and Developmental Biology, Tokyo Dental College, Tokyo, Japan, Division of Biotechnology and Convergence, Daegu
Haany University, Gyeongsan Korea
BACKGROUND AIM
Tongue is centrally located in oral cavity with its In this study, we examined the precise
dorsal surface covered with plenty of the specialized papillae, expression pattern and developmental function of YAP
which accommodate the taste sensation including fungiform, to define the fundamental signaling regulations in CVP
foliate, and circumvallate. Though circumvallate papillae morphogenesis. Particularly, we examined the
have been extensively studied to determine the regulatory involvement of YAP in development and homeostasis of
mechanisms during histomorphogenesis and homeostasis, it taste bud by clarifying the immunohistochemistry
still remains to be elucidated the principle mechanisms of localization patterns of YAP and the related markers in
signaling regulations on morphogenesis. Yes-associated taste buds within circumvallate papillae. Overall, we
protein 1 (YAP1) is the key regulator of the Hippo pathway, establish the reciprocal mechanisms in regulating the
which is mainly involved in regulations of tissue homeostasis, formation and maintenance of taste bud by comparing
organogenesis, regeneration and tumorigenesis. the localizations of YAP with those signaling effectors.
METHODS

Animal: The adult ICR mice were housed by group under given condition including room temperature 22 ± 2 °C, humidity of 55 ± 5 %, and artificial
illumination which lights on from 05:00 to 17:00 hours. The day on which a vaginal plug was confirmed was designated as E0. Mouse embryos at E16 and
male mouse (8 weeks old) were used in this study.
Tissue preparation: The preparation were performed as previously described (Adhikari et al.,2018). The sections were prepared for hematoxylin and
eosin (H&E) and immunohistochemistry staining.
Immunohistochemistry: Immunohistochemistry were performed as previously described (Adhikari et al.,2018). Following primary antibodies: Rabbit
polyclonal antibodies against Ki67, YAP, β-catenin, Cytokeratin (CK8) 8 and CK14, Rabbit monoclonal to p-YAP and non-phospho (Active) β-catenin,
and mouse monoclonal PGP9.5.
RESULTS

Hippo pathway and related Wnt /β-catenin Figure. 2 Immunolocalization of β-catenin and PGP9.5 in CVP. (A1-A3) , Localization pattern of β-
catenin in developing CVP, (B1-B4). (C1-C3) At E16, localization pattern of non-phospho β-catenin
(D1-D4) and adult.. (E1-E3) Localization pattern of PGP9.5 at E16 and (F1-F4) adult CVP.

Figure. 1 Histogenesis of CVP and Taste buds. (A1-A3) HE staining of developing CVP. (B1-B4)
HE staining of adult CVP. (C1-C3) Localization pattern of Ki67 at E16, (D1-D4) Localization Figure. 3 Immunolocalization of CK8 and CK14 in CVP. (A1-A4) Localization pattern of
pattern of Ki67 at adult CVP. (E1-E3) At E16, localization pattern of YAP, (F1-F4) and adult CK8 in adult CVP. (B1-B4) At adult, localization pattern of CK14.
CVP.(G1-G3) Localization pattern of p-YAP in developing CVP, (H1-H4) and adult CVP.
CONCLUSION REFERENCES ACKNOWLEDGEMENTS

• Maintenance of taste bud in CVP Adhikari N et al., 2018. PMID: 29181608 This work was supported by a grant from the
National Research Foundation of Korea (NRF)
epithelium Asano-Miyoshi et al., 2008. PMID: funded by the Korean government (grant no. NRF-
• Hippo signaling path way for 17960487 2019R1I1A3A01062091).
taste bud formation and Barlow LA. 2015. PMID: 26534983 Contact information
maintenance in CVP Chen Y-A et al., 2019. PMID: 30805310
• Signaling regulations for Hansen C et al., 2015. PMID: 26045258 Correspondence: Jae-Kwang Jung (Email:
widenmy@knu.ac.kr) Jae-Young Kim (Email:
epithelial differentiation Kim JY et al., 2009. PMID: 19014928 jykim91@knu.ac.kr)
Kim M and Jho EH. 2014. PMID: 25154721
through Wnt and Hippo in CVP Lange A et al., 2015. PMID: 25480985 , School of Dentistry, IHBR, Kyungpook National
University, 2177 Dalgubeol-daero, Joong-gu, Daegu
41940, Korea