Page 82 - I. Chemical biology and drug discovery

[I. Chemical biology and drug discovery-44]

Target analysis of ingredients included in herbal remedies

related to type 2 diabetes by high-throughput screening and

network analysis

Youllee Kim¹, Dohee Ahn¹, Sang J. Chung¹˙*

¹School of pharmacy, Sungkyunkwan University, Suwon 16419, South Korea

Traditional oriental medicine (TOM) has been used to treat disease for thousands of years. However, the targets of
TOM have been barely validated by modern analytical science because TOM has been established by qualitative
and philosophical approach. To create the method which reveal the target of TOM ingredients, we have accumulated

a set of biological data based on in vitro enzyme assay with high-throughput screening of 658 purified natural
products for inhibition of human protein tyrosine phosphatase (PTP) libraries consisting of 77 members. The

resulting data were combined with the data of herbal medicine, which affords pre ready data for systemic analysis
of disease, medicinal target, natural product, herbal medicine, herbal remedies for type 2 diabetes (T2D). Ingredients

included in the diabetes-related herbal remedies have been shown to inhibit diabetes-related PTPs. Among herbal
medicine prescriptions for T2D, the most targeted PTP was PTPN9 (MEG2). This is the first example that a set of

prescriptions for a specific disease was validated by modern analytical science. The result supported that TOM has
each medicinal target for specific disease. This type of analysis may pave a way to validate TOM and to discover

novel medicinal targets at the same time.