Page 86 - I. Chemical biology and drug discovery

[I. Chemical biology and drug discovery-50]

Production process development of an Engineered EGF

(Epidermal growth factor) protein

Youngjae Jeon¹, Hyung-tak Jin², Yunsik Choi¹

¹Research Institute, SLBiGen, Inc., Incheon 21983, Korea, ²Research Institute, ProGen, Inc. , Seongnam 13488, Korea

EGF (epidermal growth factor) was generally known to be one of the major factors for maintaining the skin
homeostasis and recovering injured states of skin. Therefore, recombinant human EGF have for a long time been
applied to cosmetics or medical device for improving skin conditions. However, there are still a few limitations for

EGF to be used with an expected efficacy in vivo, in which recombinant human EGF is known to be not stable in a

solution and its commercial price is relatively very expensive. To solve these limitations, we made a cell line
expressing the engineering EGF (EGF-hyFc). Here, we showed that production process development of EGF-hyFc
was successfully finished with ≥3.0g/L culture productivity and ≥65% of purification yield. And also EGF-hyFc was

stable in a severe temperature condition of 40ºC when incubated more than 3 months.