Page 26 - H. Cell signaling

[H. Cell signaling-20]

Gallotannin regulates differentiation by changing Actin

cytoskeleton and cell morphology in Human osteoblast-like

Saos-2 cells

Byung Su Ko¹, Song Ja Kim¹˙*

¹Department of Biological science, Kongju National University, Gongju 32588, Republic of Korea

Gallotannin (GT) is a class of polyphenols with antioxidant, anticancer, and antiviral activities. GT in primary-cultured
chondrocytes enhances expression of type ii collagen, an indicator of differentiation. However, the effect of
differentiation is not clear in Human osteoblast-like Saos-2 cells. Therefore, we have investigated the effects of GT

on differentiation of Saos-2 cells. The results showed that type Ⅱ collagen expression was increased on 0, 1, 3, 10
µM through western blot analysis in Saos-2 cells. Also, aggrecan expression was increased on 0, 1, 3, 10 µM. But,

after 10 µM decreased type Ⅱ collagen and aggrecan expression. In addition, GT changed of Actin cytoskeleton
and cell morphology. Based on immunofluorescence staining, expression of actin cytoskeleton was increased by GT

10 µM treated. However, expression of actin cytoskeleton was reduced on 50 µM. This results that GT regulates
differentiation by changing actin cytoskeleton and cell morphology in Human osteoblast-like Saos-2 cells.