Page 34 - F. Cell biology

[F. Cell biology-21]

Effects of PCB 126 on PKC Activation and Kv1.5 and Kv1.3

Channel Current

Jong-hui Kim¹˙²˙*, Soobeen Hwang¹˙²˙*, Su-Hyun Jo¹˙²

¹Interdisciplinary Graduate Program for BIT Medical Convergence, Kangwon National University, Chuncheon 200-

701, Korea , ²Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 200-701,
Korea

PCBs have been known as serious persistent organic pollutants, causing developmental delays and motor

dysfunction. we investigated the effects of PCB126. We observed the phosphorylation of PKC with signal

transduction, cell growth, and gene expression by western blotting. We treated ventricular myocytes from guinea
pig with 0.1 ~ 100 µM of PCB126 for 15 ~ 60 min. The phosphorylation of αβⅡ decreased significantly by 20% at
1 µM of PCB126. Also, PCB 126 increased the phosphorylation of the PKCr by 180% at 1 ~ 100 µM. However,

PCB126 did not change the phosphorylation of PKCε and PKCz at all concentrations of we tested. Also, we
conducted further studies on the changes of Kv1.5 and Kv1.3 channel current by PCB126. We expressed the Kv1.5

channels, which contributes to action potential repolarization of myocytes in heart, and Kv1.3 channels, which
contributes to expresse immune cell like to T lymphocytes, in Xenopus oocytes and treated PCB126 (10 µM~100

µM) for 8 minutes and 15 minutes. We observed that the peak and steady state current of Kv1.5 and Kv1.3 channel
did not change. The present data indicate that PCB 126 could affect cardiac function possibly by activation of PKC.

There results suggested that change of PKC phosphorylation did not modulate the Kv channel function.