Page 32 - F. Cell biology

[F. Cell biology-20]

Effects of PCB 19 on Kv1.3 Channel currents

Jong-hui Kim¹˙², Su-Hyun Jo¹˙²

¹1Interdisciplinary Graduate Program for BIT Medical Convergence, Kangwon National University, Chuncheon

200-701, Korea , ²Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 200-
701, Korea

Polychlorinated biphenyls consist of two six-carbon rings linked by a single carbon bond and theoretically 209

congeners can form, depending upon the number of chlorines and their location on the biphenyl rings. PCBs have

been classified into dioxin-like (DL) and non-dioxin-like (NDL) groups. PCB19, a 2,2’6-trichlorinated biphenyl is one
of many non-dioxin-like polychlorinated biphenyls (NDL PCBs), which are ubiquitous pollutants. NDL PCBs perturbed
functions of cerebellar granule cells, PC12, Jurkat T, HL-60, HEK293 cells, membrane vesicles, and uterine cells. We

studied the effect PCB19 on Kv1.3 channels, the one immune-modulating mechanism and the voltage-dependent
potassium channel, expressed in Xenopus oocytes. In this study, PCB19, NDL PCB, with no aryl hydrocarbon receptor

affinity blocked peak currents of the Kv1.3 channel and identify that inhibited the currents in a concentration-
dependen. Also, peak current of Kv1.3 irreversible suppressed of Kv1.3 channel by PCB19, this indicates that PCB19

did not wash out. These results suggest the possibility that PCB19 could inhibit the immune mechanism by
suppressing the current of the Kv1.3 channel and suggest the study about more PCB congeners present the necessity.