Page 26 - F. Cell biology

[F. Cell biology-17]

Decrease in lipid droplet formation by the target in NIH3T3-

L1 cells

Anh-Thu Nugyen-Hoang¹, Long Ngo-Hoang¹, Sook-Jeong Lee¹

¹Bioactive Material Science, Jeonbuk National University, Jeonju 54896, South Korea

Obesity is characterized by an increase in the adipose tissue mass and is associated with the incidence of obesity-
related conditions including hypertension, heart disease, type 2 diabetes mellitus (T2D), and cancer. In obesity, the
level of circulating factors, including insulin, adipocytokines and fatty acids as well as the lipid deposition in the

liver and skeletal muscles, are altered. The target we found, which is one of the serine/threonine protein kinase

family, plays an important role in eukaryotic signaling pathways, and their substrates are essential regulatory proteins
involved in cell differentiation, proliferation, chromosome segregation, and cell biorhythms. The target protein has
been investigate as promising target for discovery of anti-trypanosomal drugs. 3T3-L1 cells, which are murine pre-

adipocytes, can be differentiated into mature adipocytes within two weeks using a combination of insulin,
dexamethasone and isobutylmethylxanthine. In this research, we have established a model, which overexpresses a

number of mature adipocytes by using a fatty acid (oleic acid). Then, we found that inhibition of the target
significantly reduces the formation of lipid droplets in fully differentiated adipocytes.