Page 25 - F. Cell biology

Decrease in lipid droplet formation by the target
in 3T3-L1 cells
1
1
Anh-Thu Nguyen-Hoang ; Hoang-Long Ngo ; Sook-Jeong Lee 1
1 Department of Bioactive Material Science, Jeonbuk National University, Jeonju, South Korea
BACKGROUND AIM

Obesity is characterized by an increase in the adipose tissue mass The serine/threonine protein kinases play an important role
and is associated with the incidence of obesity-related conditions in eukaryotic signaling pathways, such as cell differentiation,
including hypertension, heart disease, type 2 diabetes mellitus (T2D), proliferation, and cell metabolisms. Therefore, we
and cancer. In obesity, the level of circulating factors, including insulin, experimented to find the target which are effective for
adipocytokines, and fatty acids as well as the lipid deposition in the liver reduction in the lipid droplet formation in 3T3-L1 cells.
and skeletal muscles, are altered.

METHODS

Purpose of Culture Media types
3T3-L1 cells
1 Before the differentiation DMEM
10% Calf serum
2 Differentiation DMEM
10% Fetal Bovine Serum
0.5 mM IBMX (M)
1 μM Dexamethsome (D)
10 μg/ml Insulin (I)
3 After the differentiation DMEM
10% FBS
10 μg/ml Insulin

RESULTS

Figure 1. Oleic acid induces the adipogenesis in 3T3-L1 cells. Figure 2. Target inhibitor reduces oleic acid-induced adipogenesis in
3T3-L1 cells.
(A) (A) 10 Vehicle **
8 T-Inhibitor *
Lipid droplets 6 4 *

(B) 2
0
Preadipocytes 0 250 500
Oliec acid [M]
(C) 20 D8 *** (B)
D6 **
D4
15 D2
D0
Lipid droplets 10 5 *

0
0 250 500
Oleic acid [M]
(A-B) Microscopy of lipid droplets of 3T3-L1 preadipocyte and differentiated
adipocytes on day 1 to day 8 after Oil red O staining. (size bars, 100 μm) (C) (A) Measurement of lipid droplets after oleic acid alone or combined treatment
Bars denote the quantitative analysis of the number of lipid droplets formed with a target inhibitor. (B) Microscopy of lipid droplets of 3T3-L1 preadipocyte and
after 250 and 500 μM of oleic acid treatment. differentiated adipocytes on day 8 after Oil red O staining (size bar, 100 μm).
CONCLUSION REFERENCES ACKNOWLEDGEMENTS
1. Malgorzata Malodobra-Mazur et al. Oleic acid This work was supported by Basic
influences the adipogenesis of 3T3-L1 cells via Science Research Program through the
This study shows that oleic acid affects DNA Methylation and may predispose to obesity
the lipid droplet formation in 3T3-L1 and obesity-related disorders. Lipid Health Dis. National Research Foundation of Korea
cells. The target inhibitor found by us 2019; 18: 230. (NRF) funded by the Ministry of
greatly decreases oleic acid-triggered 2. Yong Wu et al. Activation of protein phosphatase Education (Grant no. NRF-
lipid droplets in 3T3-L1 cells, 2A by palmitate inhibits AMP-activated protein 21016R1D1A1B04934383).
suggesting of a possible clinical use kinase. J Biol Chem. 2019 Jul 5;294(27):10741.
for ketogenesis. 3. Korta, T. A. et al. Regulation of lipid accumulation in Contact information
3T3-L1 cells: insulin-independent and combined
effects of fatty acids and insulin. Animal (2008, 2:1, Anh-Thu Nguyen-Hoang:
pp92-99). anhthu232@gmail.com