Page 90 - D. Cancer biology

[D. Cancer biology-59]

Downregulation of ACSM3 promotes tumorigenesis in breast

cancer

HONG BEUM KIM¹

¹Department of Premedical Course, Chosun University School of Medicine, Gwangju 61453, Republic of Korea

Breast cancer is one of the most common cancer diagnosed worldwide. However, the detailed molecular
mechanisms underlying antitumor activity in breast cancer remains largely unknown. Acyl-CoA medium-chain
synthetase 3 (ACSM3) is an acyl-CoA synthetase which takes part in the first step of fatty acid metabolism. We

investigated the biological function and clinical implications of ACSM3 in breast cancer. We revealed ACSM3-

mediated gene expression profile in breast cancer cells using RNA-Sequencing. We found that ACSM3 expression
was significantly decreased in tumors (75%) compared to normal. Migration & invasion assay and soft agar assays
were carried out for functional analysis in vitro and a xenograft model was used to analyze the effects of ACSM3

on cancer growth in vivo. Overexpression of ACSM3 attenuated migration and invasion of breast cancer cells in
vitro and upregulated the phosphorylation of AKT. Our study indicates that ACSM3 suppresses cell growth, migration

and invasion by directly upregulating WNT/AKT-pathway in breast cancer, acting as a tumor suppressor. Our study
also suggests that ACSM3 may serve as a potential therapeutic target for patients in breast cancer.